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#!/usr/bin/perl -w | |
my $columns = 50; | |
my $gapped = 0; | |
my $progname = $0; | |
$progname =~ s/^.*?([^\/]+)$/$1/; | |
my $usage = "Usage: $progname [<Stockholm file(s)>]\n"; | |
$usage .= " [-h] print this help message\n"; | |
$usage .= " [-g] write gapped FASTA output\n"; | |
$usage .= " [-s] sort sequences by name\n"; | |
$usage .= " [-c <cols>] number of columns for FASTA output (default is $columns)\n"; | |
# parse cmd-line opts | |
my @argv; | |
while (@ARGV) { | |
my $arg = shift; | |
if ($arg eq "-h") { | |
die $usage; | |
} elsif ($arg eq "-g") { | |
$gapped = 1; | |
} elsif ($arg eq "-s"){ | |
$sorted = 1; | |
} elsif ($arg eq "-c") { | |
defined ($columns = shift) or die $usage; | |
} else { | |
push @argv, $arg; | |
} | |
} | |
@ARGV = @argv; | |
my @seqorder = (); | |
my %seq; | |
while (<>) { | |
next unless /\S/; | |
next if /^\s*\#/; | |
if (/^\s*\/\//) { printseq() } | |
else { | |
chomp; | |
my ($name, $seq) = split; | |
$seq =~ s/[\.\-]//g unless $gapped; | |
push @seqorder, $name unless exists $seq{$name}; | |
$seq{$name} .= $seq; | |
} | |
} | |
printseq(); | |
sub printseq { | |
@seqorder = sort @seqorder if $sorted; | |
foreach $key (@seqorder) { | |
print ">$key\n"; | |
for (my $i = 0; $i < length $seq{$key}; $i += $columns){ | |
print substr($seq{$key}, $i, $columns), "\n"; | |
} | |
} | |
%seq = (); | |
@seqorder = (); | |
} |
2 comments:
hi, do you have a fasta 2 stockholm format converter?
https://www.google.com/search?q=fasta2stockholm.pl ?
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